KMID : 1240020150190010012
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International Neurourology Journal 2015 Volume.19 No. 1 p.12 ~ p.18
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Role of Nicotinic Acetylcholine Receptor ¥á3 and ¥á7 Subunits in Detrusor Overactivity Induced by Partial Bladder Outlet Obstruction in Rats
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:Kim Hyo-Sin
:Park Wang-Jin/:Park Eun-Young/:Koh Jun-Sung/:Hwang Tae-Kon/:Kim Joon-Chul
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Abstract
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Purpose : To investigate the role of ¥á3 and ¥á7 nicotinic acetylcholine receptor subunits (nAChRs) in the bladder, using a rat model with detrusor overactivity induced by partial bladder outlet obstruction (BOO).
Methods : Forty Sprague-Dawley rats were used: 10 were sham-operated (control group) and 30 were observed for 3 weeks after partial BOO. BOO-induced rats were further divided into 3 groups: Two groups of 10 rats each received intravesicular infusions with hexamethonium (HM group; n=10) or methyllycaconitine (MLC group; n=10), which are antagonists for ¥á3 and ¥á7 nAChRs, respectively. The remaining BOO-induced rats received only saline infusion (BOO group; n=10). Based on the contraction interval measurements using cystometrogram, the contraction pressure and nonvoiding bladder contractions were compared between the control and the three BOO-induced groups. Immunofluorescent staining and Western blotting were used to analyze ¥á3 and ¥á7 nAChRs levels.
Results : The contraction interval of the MLC group was higher than that of the BOO group (P<0.05). Nonvoiding bladder contraction almost disappeared in the HM and MLC groups. Contraction pressure increased in the BOO group (P<0.05) compared with the control group and decreased in the HM and MLC groups compared with the BOO group (P<0.05). Immunofluorescence staining showed that the ¥á3 nAChR signals increased in the urothelium, and the ¥á7 nAChR signals increased in the urothelium and detrusor muscle of the BOO group compared with the control group. Western blot analysis showed that both ¥á3 and ¥á7 nAChR levels increased in the BOO group (P<0.05).
Conclusions : Alpha3 and ¥á7 nAChRs are associated with detrusor overactivity induced by BOO. Furthermore, nAChR antagonists could help in clinically improving detrusor overactivity.
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KEYWORD
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Urinary Bladder, Overactive, Alpha7 Nicotinic Acetylcholine Receptor, Urinary Bladder Neck Obstruction
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